Chinese researchers have pioneered a revolutionary new generation of multicolor miniaturized two-photon microscope, weighing a mere 2.6 grams. This groundbreaking device marks the first time that high-resolution, multicolor imaging of the brains of freely moving mice has been achieved. The microscope leverages an innovative ultra-broadband hollow fiber technology, capable of transmitting a spectrum of femtosecond lasers spanning the wavelength range from 700 to 1060 nanometers. This advanced technology enabled the research team to simultaneously capture dynamic three-color images of neuronal calcium signals, mitochondrial calcium signals, and plaque deposition in a mouse model of Alzheimer's disease. Remarkably, these images were captured at a depth exceeding 820 micrometers without causing any damage to the brain tissue. This significant advancement offers a novel and powerful tool for exploring brain function, unraveling the mechanisms of brain diseases, and evaluating neuropharmaceuticals.